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2019/10/11 15:52:17

E.coli Topoisomerase IV Decatenation Assay Kits

除用于脱碳反应的分析和稀释缓冲液外,这些物质还含有酶和连接的kDNA底物。1 Utopo IV将在1X缓冲液中孵育,总反应量为30 l, 37 C,孵育30分钟,脱羧200ng kDNA
These contain the enzyme and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes.
Cat No. Product Product description
D4001 E. coli Topo IV Decatenation Kit 1
 
100 U of E. coli Topo IV and 20 µg kDNA
D4002 E. coli Topo IV Decatenation Kit 2
 
500 U of E. coli Topo IV and 100 µg kDNA
D4003 E. coli Topo IV Decatenation Kit 3
 
1000 U of E. coli Topo IV and 200 µg kDNA
D4004 E. coli Topo IV Decatenation Kit 4
 
2000 U of E. coli Topo IV and 400 µg kDNA
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