95%纯度。topoIV在稀释...">

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2019/10/14 11:33:21
铜绿假单胞菌topo IV是通过在大肠杆菌中过表达亚基,并通过自行开发的方法进行纯化而制备的。亚基经SDS-PAGE纯化至>95%纯度。topo IV在稀释缓冲液中作为异四聚体复合物提供。建议重新引用酶,以避免重复的冻融循环。储存于-80℃。所有酶都提供5X浓缩的分析缓冲液和稀释缓冲液,也可单独使用。

Pseudomonas aeruginosa topo IV is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
优势供应产品:
Cat No. Product Product description
PAT4001 P. aeruginosa Topo IV
 
100 U
PAT4002 P. aeruginosa Topo IV
 
500 U

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