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2019/10/12 14:57:07
Inspiralis提供T4 DNA连接酶已有多年。我们可以少量供应,也可以大量供应,或者更集中供应(例如,用于dna标记化学图书馆建设)T4 DNA连接酶催化磷酸二酯键在末端5磷酸和3个双链DNARNA羟基之间形成。它可以连接DNA或寡核苷酸的粘性末端,以及RNARNA-DNA的杂交体,在双链DNA上尤其有效。它也可以连接钝端DNA,但不能连接单链核酸。它需要ATP作为辅助因子。T4 DNA连接酶以2000 u /ul13000 u /ul的速度供应,并提供10X缓冲。1U是一个内聚的末端连接单元(CELU)650CELUs大约是一个Weiss单元,所以2000CELUs/ul相当于~3Weiss单元/ul (TDL1, TDL2)20Weiss单元/ul (TDL3C)
Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).
T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA.  It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.
T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)

优势供应产品:
CatNo. Product
TDL1 Kit 1
80,000U T4 DNA ligase (2000U/ul), 10X buffer
TDL2 Kit 2
400,000U T4 DNA ligase (2000U/ul), 10X buffer
TDL3C Kit3
400,000U T4 DNA ligase (13,000U/ul), 10X buffer

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