太阳集团城娱8722优势供应:T4 RNA Ligase 1(T4 RNA连接酶1)
2019/10/12 14:53:05
T4 RNA连接酶1催化在单链RNA或DNA的末端5个磷酸和3个羟基之间形成磷酸二酯键。它需要ATP作为辅助因子。一个单位被定义为将50%等摩尔(125nM)的单链5 fam标记的17-mer RNA连接到5个磷酸化的18-mer RNA上所需要的酶量,20 L, 37 C, 30分钟。
T4 RNA Ligase 1 catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of single-stranded RNA or DNA. It requires ATP as a cofactor.
One unit is defined as the amount of enzyme required to ligate 50% of an equimolar mix (125nM) of a single-stranded 5′ FAM-labeled 17-mer RNA to a 5′ phosphorylated 18-mer RNA in 20 µL at 37°C for 30 minutes.
优势供应产品:
Cat No. | Product |
TRL101 | Kit 1 2,000U T4 RNA ligase 1 (20U/uL; 100uL), 10X buffer |
TRL102 | Kit 2 10,000U T4 RNA ligase 1 (20U/uL; 500uL), 10X buffer |